Journal: Redox Biology
Article Title: Perinatal inflammation alters histone 3 and histone 4 methylation patterns: Effects of MiR-29b supplementation
doi: 10.1016/j.redox.2020.101783
Figure Lengend Snippet: Western blot analysis of matrix proteins. Lung tissues were harvested at PN28 to assess levels of matrix remodeling proteins, αSMA (A) and MMP-9 (B), and pulmonary signaling proteins, PDGFα (C) and its cognate receptor PDGFRα (D), via Western blot. Data were quantified by densitometry and analyzed by two-way ANOVA with Tukey's post-hoc; n = 6–8.
Article Snippet: After blocking, membranes were probed with antibodies to αSMA (Sigma #A2547), MMP-9 (Abcam #ab119906), PDGFα (ABBiotech #250814) and PDGFRα (Cell Signaling #3164s) to assess changes in matrix proteins and PRMT1 (Cell Signaling #2449) and PRMT5 (Cell Signaling #2252) to assess arginine methyltransferases.
Techniques: Western Blot